BIOLOGY 1A03 Lecture Notes - Lecture 4: Transcription Bubble, Molecular Machine, Ribonucleoside

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Rosada Silva

Document Summary

Theme 2: blueprint of a cell; module 1: transcription. Learning objectives: describe the basic mechanism of transcription in prokaryotes and eukaryotes. Identify how rna polymerase is able to create an rna transcript from a dna template. Illustrate how messenger rnas are processed in eukaryotes: explain how compartmentalization of eukaryotic cells allows for transcription and functional maturation of mrna prior to translation in the cytosol. Central dogma: dna to rna to proteins, transcription then translation. Dna has a start and end site: an enzyme called rna polymerase attaches to specific promoter regions of the dna. September 26 2014: many consensus sequences, such as the ttgcca sequence, approximately 35 nucleotides upstream, of the transcription site. Once rna polymerase binds to the specific promoter regions of dna, it is able to open up the double-stranded dna, revealing the template and non-template strand within the transcription bubble.