BIOLOGY 2B03 Lecture 6: Bio 2B03 Module 3 Lecture 2

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19 Oct 2016
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With the human eye we can see some nerve cells and the single cell of some eggs. If something is smaller than a mm we would need to use a microscope. We can use a light microscope to see prokaryotic cells and the organelles of eukaryotes. Anything less than 100nm we would need to use an electron microscope which shoots beams of electrons to allow us to visualize small bacteria, viruses and protein complexes like ribosomes or large proteins, lipids or small molecules. Resolution: the smallest distance between two objects at which the objects still appear. A smaller d value means the image will be clearer and have more detail. To calculate d we need the wavelength of the illuminated light and the numerical aperture (na) which is a measure of the ability to gather light and resolve specimen detail. A smaller wavelength of light means a smaller d value and greater resolution.

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