BIOLOGY 2B03 Lecture Notes - Lecture 10: Metaphase, Condensin, Telophase
11 Dec 2015
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Document Summary
Interphase prophase: chromosomes condense, nuclear envelope breaks down, organelles fragment, mitotic spindle assembles, all initiated by phosphoregulation of key proteins mediated by active. Active mpf: heterodimer of mitotic cyclin and cdk. End of mitosis: mpf can coordinate these changes to happen at the same time, all changes have to be reversed, requires inactivation of mpf through ubiquitin-mediated degradation of. Cyclin b: phosphatases then reverse the phosphorylation of the same key proteins initiates chain of events to ensure cell is reset to interphase. Specific proteins phosphorylated cascade of events leading to change in behaviour. Mpf triggers: formation of mitotic spindle phosphorylation of microtubule- associated proteins. End of telophase: nuclear envelope reforms, chromosomes decondense, mitotic spindle disassembles, membranes of the golgi and er reassemble. Dna stain dapi: chromatin dispersed throughout nucleus in interphase, chromosomes highly compacted in mitosis individual chromosomes distinguishable. Arms are separated apart from one another slightly. Targets of mpf: condensins, cohesins, histones (h1,h3), topoisomerase.
