BIOLOGY 2B03 Lecture Notes - Lecture 7: Actomyosin Ring, Filopodia, Sarcomere
29 Jan 2016
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3 classes of filaments by diameter & type of subunit building block filament when bound. Label w antibody/ gfp: microtubules: antibody specific to tubulin subunits ex: tubullin gfp, intermediate filaments (ifs) : antibody gfp labet to monomeric subunit. Microtubles : thickest (alpha & beta tubulin) Actin form shape of microvilli at cell surface. If (support) & structure + shape to nucleus. Filament specific motor protiens to actin/microtubules not if: myosin (actin, kinesin & dynein (microtubules, use cycling chem rxn, head domain bind to filament & tail attach to cargo. Cleft b/t 2 & 4 for atp bindingsite. Critical conc: conc rate of addition= rate of removal, no net change: if conc of actin monomer > crit conc = polymer>deploy, if conc actin monomer poly, crit conc diff at both ends. 100s of proteins; profilin -> bind actin atp ->promote atp binding & activating monomer (a lot at plus end)
