BIOLOGY 2D03 Lecture Notes - Lecture 3: Vascular Cambium, Reporter Gene, Phloem
28 Mar 2018
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Endoreduplication: 4c = 4 copies (2 rounds of mitosis, no nuclear or cell division. Important to allow cells to grow: reduced endoreduplication, results in a small plant. Primary cell wall: major contributor in dictating plant cell shape. Strength to withstand the outward pressure of water on plasma membrane (turgor pressure) Must be very strong allowing structural integrity, but also must be flexible. If you do(cid:374)"t ha(cid:448)e good (cid:272)ellulose microfibrils, the cell wall is not strong enough to resist turgor pressure. How do cell wall components interact to form a strong yet flexible structure. Microtubules contribute to where cellulose microfibrils are laid down: reporter gene (can be seen, fused to gene of interest using confocal microscopy, 260 seconds later see cesa 6 forming a microfibril, results summarized on slide* Instead of using a mutant, a drug was used to disrupt microtubule function. Sever microtubule mutant would stop it from working. Evidence that microtubules are required to direct cell expansion.
