BIOLOGY 2F03 Lecture Notes - Lecture 1: Biuret Test, Heat Capacity, Nuclear Pore

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Topics to Know Gr. 12 Biology
Isotopes/Radioisotopes
Organic Molecules
Inter/Intramolecular Forces (ionic, Covalent, Hydrogen, Hydrophobic, hydrophilic, electrostatic
attractions)
Functional Groups
4 Types of Macromolecules (Structure, monomers, polymers, bonding, function)
Monosacchride Isomers (glucose, fructose, galactose /ribose)
Disacchrides (lactose, sucrose, maltose)
Polysacchrides (Starch, Glycogen, Cellulose, Chitin, pectin)
Lipid/Phospholipid (mono/poly/unsaturated structures)
Steroid/Waxes structure and function
Proteins (glycerol, levels of protein organization, Functions)
Nucleic Acids (DNA vs RNA, nucleotides, structure)
4 Biochemical reactions (neutralization, redox, dehydration, hydrolysis)
Enzyme-Substrate Complex (active site, allosteric, inhibitor, activator, feedback inhibition)
Optimal Enzyme Conditions/Denaturation (Catalase H202-> CO2 gas production lab)
Identify Macromolecule Experiment
-Monosacchride (Benidicts solution, heat Blue(-) Orange (+)
-Polysacchride (Iodine solution Dark Red (-) Black (+)
-Proteins (Biuret reagent Blue (-) Violet/Black (+)
-Lipids (Ethanol, clear(-) White precipate (+)
Properties of Water (universal solvent, cohesion, adhesion, density of ice, hydrogen bonding/polarity,
specific heat capacity, boiling/melting point)
Parts of the Cell (Cytoskeleton, Microtubules, Microfilaments, intermediate filaments,lysosomes ,
peroxisomes, centrioles, golgi, ER, ribosomes, chloroplast, mitochondria,cilia, flagella)
Parts of the nucleus (nuclear pore, envelope, lumen, inner membrane, chromatin, chromosome, nuclear
matrix, nucleoplasm)
Endomembrane System (Nucleus DNA Transcription, Strand goes from Lumen to Rough ER, ribosome
translation to protein, Smooth ER makes lipids,steroids,hormones, ER makes vesicles to Golgi, packages,
secretory vesicle, exocytosis: incoming vesicle, lysosome breaks down, Golgi packages/resuses)
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Document Summary

Inter/intramolecular forces (ionic, covalent, hydrogen, hydrophobic, hydrophilic, electrostatic attractions) 4 types of macromolecules (structure, monomers, polymers, bonding, function) Enzyme-substrate complex (active site, allosteric, inhibitor, activator, feedback inhibition) Optimal enzyme conditions/denaturation (catalase h202-> co2 gas production lab) Polysacchride (iodine solution dark red (-) black (+) Properties of water (universal solvent, cohesion, adhesion, density of ice, hydrogen bonding/polarity, specific heat capacity, boiling/melting point) Parts of the cell (cytoskeleton, microtubules, microfilaments, intermediate filaments,lysosomes , peroxisomes, centrioles, golgi, er, ribosomes, chloroplast, mitochondria,cilia, flagella) Parts of the nucleus (nuclear pore, envelope, lumen, inner membrane, chromatin, chromosome, nuclear matrix, nucleoplasm) Endomembrane system (nucleus dna transcription, strand goes from lumen to rough er, ribosome translation to protein, smooth er makes lipids,steroids,hormones, er makes vesicles to golgi, packages, secretory vesicle, exocytosis: incoming vesicle, lysosome breaks down, golgi packages/resuses) Peroxisome (oxidase enzymes) lysosome (hydrolysis enzymes)- different ph from rest of cell protection. Parts of chloroplast (stroma, thylakoids, grana, inner/outer membranes)

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