BCHM 218 Lecture Notes - Lecture 19: Gentamicin, Arginine, Bactericide

• School: Queen's University
• Department: Biochemistry
• Course: BCHM 218
• Professor: Mark Ormiston

Document Summary

Short nucleic acids containing 50 or less nucleotides are called oligonucleotide, long= polynucleotide. Self-assemble by weak forces (wastson crick base pairs + base stacking) = most e fav structure. Nucletodies= heterocyclic base, 5 c sugar, p group: purines: ag double ring, pyr: ct single ring numbering convention. Sense st(cid:396)a(cid:374)d: (cid:1009)(cid:859)-atg-(cid:1007)(cid:859) (top strand written in 5-3) Equal % of purines and pyrimidines bc a pairs w t and g=c (3 h bonds) Backbone= alt sugar and p residues (sugar-p backbone), -vely charged. Nitrogenous bases twrds center so they can h bond w bases on opposing strand. Each strand has opposing directionality, one is 5-3 other is 3-5 (antiparallel which is more e fav) (cid:1009)(cid:859)p li(cid:374)k (cid:449) (cid:1007)(cid:859)oh of (cid:374)e(cid:454)t (cid:374)u(cid:272)leotide, loss of (cid:449)ate(cid:396) aa (cid:374)o(cid:449) (cid:272)alled (cid:396)esidues. Small changes= significant effect bc its highly conserved. Stab factors can be internal or external.