BCHM 310 Lecture Notes - Lecture 4: C-Terminus, N-Terminus, Isoelectric Point
22 Dec 2018
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Isoelectric point: the ph where net charge=0, pi. Calc by averaging the 2 pk values surrounding that state pi= (pkr + pk2)/2. At phpi (raise the ph) the net charge is -ve. Peptides peptide bond formed by condensation or broken w hydrolysis. Draw amino terminus on l side, cooh on right. Write seq left to right from amino to carboxyl terminus. Residue: aa in the middle of chain, whats left after water has been removed. Protein: a polypeptide(s) that are products of gene transcription of mrna translation, only l aa here. No branching= linear polymer (in terms of backbone) No restrictions on seq (ex can have a pro after ala or whatever) No restrictions on aa composition: some proteins rich in certain aa ex collagen for gly, pro, others have more uniform comp. Q: histones bind to dna to form nucleosome.
