BCHM 310 Lecture 33: BCHEM 315 12:1
22 Dec 2018
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Document Summary
Acid/base catalysis: act of oh nu, protonation of lg, stab of o- of i. Cleaves viral polyproteins into indiv proteins: about a dozen distinct cleavage sites. Drug target (along w reverse transcriptase + integrase) Hiv encodes its own protease to perform these cleavages. Dozens of seq that are rec + cut (ie substrate seq) P1 is often phe but also tyr, leu, met, trp or asn (so enzyme dn make intimate contact w all of the side chains) P1" can be a pro, phe, tyr. Inhibitor example- lopinavir two mimics of phenylalanine, the c-oh group mimics. Inhibitors have properties to mimic substrate recognition sites and to mimic ts or intermediates rather than substrate conform. Important for regulating metabolism + signalling: metabolism: cells need to respond to their enviro, need to maintain a balance b/n metabolic pathways. Precursor (rearranges pathway + metabolite) intermediate metabolite final metabolite #1 and final #2.
