BIOL 334 Lecture Notes - Lecture 3: Glycolysis, Peptide, Genome
13 Jan 2018
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Document Summary
What are enzymes (enz): are a protein catalyst that speeds up the cellular chemical reaction, conditions, typically, neutral ph, physiological temperature and pressures. Each catalyst is encoded by the amino acid (a. a) gene sequence. Important: the product (p) of one enzyme is the substrate (s) for the next enz pathway. Types of metabolic pathways: linear: glycolysis, cyclic: krebs cycle, branched: amino acid synthesis, two types, divergent. Radiolabelling function: radiolabelling is used to identify and understand the distribution of a metabolic pathway, steps, tissues are pulsed" with 14c precursor, which is then followed by a non-radioactive 12c precursor. Pathway example: genome sequence (dna) > (in silico) > Open" reading frames (genes): gene sequencing (3pair id [cgc]), deduce a. a sequence (encoded protein [arg]) Proteome: type of encoded protein, also, the sum of all proteins expressed in a cell, 90% of protein encoding genes are for enz, remaining 10% used for proteins involved in:
