MICR 221 Lecture Notes - Lecture 14: Haemophilus Influenzae, Electroporation, Autolysin
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12 Nov 2016
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The uptake of dna from the medium and incorporation into the host chromosome. Cf binding stimulates the synthesis of 8-12 new proteins. It exposes a dna binding protein and nuclease on the cell surface. Dna binds is double stranded form to the binding protein. The other strand associates with proteins and crosses the cm. The one that crosses cm may integrate to form a transformant with altered genetic makeup. Double stranded dna binds and enters the cell in that form. Dna mist contain a specific 11 bp sequence for binding. Works with cells that are not naturally competent ie) e coli. Transformation of plasmids is easier than linear pieces of dna. Cells made electrocompetent: wash in cold water, competent cells are mixed with dna and exposed to high voltage pulses in electroporator. This forms small re-sealable holes in the membrane which allows dna in. *almost the universal method for cells not transformable by other methods.
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