MICR 221 Lecture Notes - Lecture 19: Tower Of Power, Ribosomal Dna, Elisa

• School: Queen's University
• Department: Microbiology and Immunology
• Course: MICR 221
• Professor: Jarrell

Document Summary

Metazoans and unicellular euk defined by repro isolation. Microbes (prokaryotes) defines via dna seq identity, >97% similarity. Horizontal gene transfer: dna transferred to diff species via transformation, transposable elements and bacteriophages, evidence- diff gc conctent + genome analysis, ford doolittle includes impact of this on tree of life web of life (nobel prize) ex. Ecoli 18% of genes are from diff species. Divergence through mutation + ns: random mutation (1/mill bp), selective p can favor mutations. Molecular clock- assumes constant mutation rate (no transposable elements)+ similar function(selective p impacts this) Sample, culture, make single isolates and identify using traditional or molecular tech (cid:894)(cid:373)ost (cid:272)a(cid:374)"t (cid:271)e (cid:272)ultured(cid:895) Shot gun clone- extract dna, pcr w universal primers (rdna folding into 16srrna), ligated to vector, seq and identify- genbank search (ccdb data base) Specific fluorescent molecular probes to identify microbes in situ. Dgge, tgge (temp grad), fame, biolog plates, genomic microarrays, next-gen seq, gram stain.