CHY 183 Lecture Notes - Restriction Enzyme, Amine, Pyrimidine

• School: Ryerson University
• Department: Chemistry
• Course: CHY 183
• Professor: Raji Iyer

Document Summary

***what is dna: stands for deoxyribonucleic acid, dna = base + sugar + phosphate. Bases = adenine, guanine, thymine, cytosine: a & g purine bases, c & t pyrimidine bases. Protein building block of amino acid. Amino acid amino group (nh2) and a carboxylic acid group (cooh: basic amino acid, glycine : h2n ch2 - cooh. Catalyst speeds up reaction without itself taking part of the reaction. Many enzymes and protein (i. e. polymerases) are involved in unwinding the dna, keeping dna strands apart, assembling new dna. ***polymerase chain reaction (pcr: technique to replicate small quantities of dna or broken pieces of dna found at a crime scene, ability to multiply small bits. ***recombinant dna replies on ability of certain chemicals, known as restriction enzymes, cuts dna into fragments: ligate is a restriction enzyme. Rflp restriction fragment length polymorphism: form the basis for one of the first dna typing procedures, core sequence (15-35 bases in length) and repeats itself up to.