BISC 202 Lecture Notes - Lecture 24: Wild Type, Insulin, Mitosis

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Bisc202 lecture 24: prokaryote genetics and mating experiments. Mapping techniques don"t work for bacteria as they don"t have a diploid stage (no meiosis) Most prokaryotes have separate events for making new recombinations (sex) and reproduction (making new cells) in comparison to eukaryotes, which is at the same time. Prokaryotic cells divide by binary fission resulting in genetically-identical daughter cells (similar to mitosis but more simple) Recall they only have one circular chromosome and sometimes some small circular plasmids. 3 ways that genes can be transferred, unidirectionally. Conjugation = donor cell forms a pilus that can attach it to a recipient cell. It can then transfer a copy of a plasmid (or even parts of the chromosome) Transformation = a recipient cell can pick up fragments of dna from the environment and incorporate pieces of it into the genome via pairs of crossover events. Transduction = when a bacteriophage (a bacteria-infecting virus) transfers some.

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