BISC 202 Lecture Notes - Lecture 25: Hfr Cell, Dna Replication, Plasmid
30 Nov 2016
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Bisc202 lecture 25: prokaryotic conjugation and high-frequency recombination. F+ cells have an f plasmid (f factor) that contains genes for recognizing f- cells and for building pili to join with them. During conjugation, 1 strand of the plasmid is peeled off and transferred into the f- cell. Dna replication allows for each single strand to become a double-helix again. This is known as a replicative transfer. Since both cells have the f plasmids, they both become f+ cells. Dna single strand enters recipient (separating from the original f-factor) Both single strands in the donor and recipient are copied into double stranded dna. The double stranded dna circularized to make a new circular f factor in the recipient. The plasmids are then replicated, along with the main bacterial chromosome every time the cell undergoes binary fission. When a single crossover occurs between the bacterial chromosome and the f plasmid in an f+ cell:
