BISC 313 Lecture Notes - Lecture 19: Biotransformation, Fugacity, Toxicokinetics

• School: Simon Fraser University
• Department: Department of Humanities Biological Sciences
• Course: BISC 313
• Professor: Chris Kennedy

Document Summary

Liver -> bile duct -> gi tract -> feces. Liver is clean up system that takes out stuff that has been absorbed by gi tract: most excretion in liver -> bile is active transport, energy! Atp: zero order kinetics, saturable, limit to rate of excretion that the liver deals with. Used rats, 4 chemicals, endpoint was lethality (ld50 value (lethal dose to 50% of animals: chem x 1mg/kg, chem y 100mg/kg, means y is less toxic, bc it takes a lot more. Ld50 (sham, bdl, sham:bdl: diethylstilbesterol (100, 0. 75, 130, digoxin (11, 2. 6, 4. 2) Idocyanin green (700, 130, 5. 4: amitriptyline (100, 100, 1. 0, purpose of this experiment was to determine importance of hbs in excretion of all of these chemicals, rats underwent surgical procedure called bdl, bile duct ligated, could not utilize. Hb excretion: control: sham, surgically operated on, not bdl, expose to chemical, determine ld50 value, start with sham group, figure out whos more toxic than the other.