MBB 322 Lecture Notes - Lecture 16: Superior Colliculus, Protein Kinase, Axon Guidance
18 Nov 2020
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Just enough sev activity to get it: did genetic screen that looked for dominant enhancer mutations. Looking for smth that makes the phenotype worse. Looking for a mutation in another gene that creates no r7 cells. Identified ras as well: measure activation of ras by fret, fret. Excited at one wavelength which results in emission at a higher wavelength: emission wavelength serves as excitation for another, violet blue, blue green, you fuse protein x and y together. If they actually interact, you"ll see green from violet bc the emission from x will be close enough to get light from y: this is how you get fluorescence resonance energy transfer. In ras: ras binds to gdp and you can link ras to yfp (yellow fluorescence protein, emits are 475 nm, gdp out, gtp bound with rfp (red flur. Cross talk : projection of retinal ganglion cells (retinal neurons) to optic tectum, dye filling experiments.
