MBB 331 Lecture Notes - Lecture 5: Bacteriophage, Ribosome-Binding Site, Pyrophosphate
22 Jul 2020
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Instead of separate test tubes, you only have one but bc it"s all labeled its gucci. Rna seq: benefits, snp short nucleotide polymorphisms. Isolate total rna: fragmented &or isolated to polyt magnetic beads (bc there"s poly a tails, beads are washed away and then fragment and reverse transcrie the rna, this mades the pcr clustered fragments, which can then be sequenced. Sds, you can run them through a electric field and matrix: 2 wells, on the left hand side, its uninduced (normal conditions) Induced on right hand side: host transduced with promotion vector, promoter induced (activated) lots of reca became available. If you"re cloning eukaryotic genes, if you clone in bacterial cells (not typical host) sometimes it doesn"t work. Includes a clamp which clamp your restriction site to pcr product, followed by a restriction site, followed by a spacer (to make sure things are translated properly), kozak sequence (initiation of translation process in eukaryotes)
