MBB 331 Lecture Notes - Lecture 7: Light Heavyweight, Base Pair, Caesium
22 Jul 2020
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If its coming from bottom of the tube, you have heavy dna. If its coming from the top, you have light dna. In conservative: you would never have any hybrids, because there"s preservation of og stands, semi-conservative, you would get a mix then your light heavy and light mix, duspersive, you would get a mix. In dsdna, they open up the dna and bring in all the machinery that"s needed in replication: terminus, part of the dna where replication stops. In circular dna: you end up with a concatemer after replication. Is it a replicon: technically speaking, yes? (though technically speaking all viruses are replicons) In this case, you would remove it from the plasmid (listen to recording: then you screen for all possibilities. Low activity label: distinguished by the intensity of the isotope firing up, then later with high radioactivity label. If replication is bidirectional, both sides will have a high intensity label.
