MBB 331 Lecture Notes - Lecture 17: Bromodomain, Dna Supercoil, Chromosome
22 Jul 2020
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Slide 7 lecture 16: chromatin packing, shorty region of dna double helix. Naked dna 2 nm: available for you to use (transcribe, translate, cut it up etc, entire mitotic chromosome. In vivo, particularly in mitosis you have something called heterochromatin: densely packed, silenced, packing, naked dna 10nm fiber. Indiv wrapped around nucleosomes (drum shaped histone octomeric core: unpacking chromatin: core particle, micrococcal endonuclease cuts dna between nucleosomes then digests linkers to release nucleosome particles, all of the interactions are non-covalent. Molecular glue : seals/attaches the double helix dna and clamps it to the octomeric core, h1 promotes condensation, chomatin: 30 nm fiber, for access, what"s important is the uniformity/distribution of the fiber. Or are there more open regions for proteins that determine how these regions are transcribed: transcriptional factors are a collective term for all the different proteins that act with.
