Class Notes (834,354)
Canada (508,493)
Biology (116)
BIOL 2000 (29)

Chapter 8-3.docx

4 Pages
Unlock Document

BIOL 2000
Joseph Rasmussen

Chapter 8-3 Clicker  Which of the following events occur during initiation of transcription by eukaryotic RNA polymerase II? o The TATA box sequence is bound by RNA polymerase II subunit protein o TFIIH bings to the TATA box sequence  TFIID is correct answer o TFIID phosphorylates to an RNA polymerase II subunit protein  TFIIH is correct o THE RNA polymerase II complex becomes catalytically active following the addition of phosphate groups to one of this subunits. o Both C and D RNA processing  All eukaryotic RNAs undergo processing events to convert a precursor(primary) transcript into its mature form Prokaryotic and eukaryotic transcription and translation  Eukaryotic- Transcription and translation are separated (nuclear barrier) o mRNAs don’t leave the nucleus until processing is complete  Prokaryotic - Couple transcription- translation o Happening in the same time RNA processing- eukaryotic nucleus  mRNA processing o 5’ cap = 7-methylguanosine added to the 5’ end o Poly (A) tail added at the 3’ end o Removal of introns and splicing together of exons Addition of 5’ cap  Very early in transcription process  Guanyltransferase enzyme adds guanosine to the 5’ end of mRNA  Must make a phosodiater bond o From three consecutive phosphates (triphosphate linkage) Practice problems: 2,3,4,8,9,11,12 Addition of a polymeric (poly) A tail  Endonuclease recognizes a sequence element o Polyadenylation signal “ AAUAAA” o Cuts the RNA transcript 3’ of this sequence  Anything further then the sequence element discarded as it is not needed  Once cleavage has occurred need enzyme that will add the ply a tail o New 3’ end when cut o Poly A polymerase will add the AAAAAAA..... Sequence Removing interrupting sequences fom mRNA  Unique to eukaryotic genes encoding proteins  Intervening seuqneces o Does not code for the functional protein  Introns- do not code o Removed after transcription  Exons= coding proteins o These sequences will be present in the final mature mRNA Splicing  Introns are transcribed along with exons in the primary transcript o RNA faithfully transcribes everything  Introns are removed and exons are spliced together. o Splicing is process of removing introns o Stitching the exons together  Splicing o Disrupting phospodiester bonds in between introns and exons o Then making new phosphodiester bonds with exons adding to exons  Must be extremely accurate or no functional protein can be made How are correct sires for splicing chosen?  Compared many genes- looking for trends in many different mRNA  Conserved sequences specify intr
More Less

Related notes for BIOL 2000

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.