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Chapter 8-3.docx

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Department
Biology
Course
BIOL 2000
Professor
Joseph Rasmussen
Semester
Winter

Description
Chapter 8-3 Clicker  Which of the following events occur during initiation of transcription by eukaryotic RNA polymerase II? o The TATA box sequence is bound by RNA polymerase II subunit protein o TFIIH bings to the TATA box sequence  TFIID is correct answer o TFIID phosphorylates to an RNA polymerase II subunit protein  TFIIH is correct o THE RNA polymerase II complex becomes catalytically active following the addition of phosphate groups to one of this subunits. o Both C and D RNA processing  All eukaryotic RNAs undergo processing events to convert a precursor(primary) transcript into its mature form Prokaryotic and eukaryotic transcription and translation  Eukaryotic- Transcription and translation are separated (nuclear barrier) o mRNAs don’t leave the nucleus until processing is complete  Prokaryotic - Couple transcription- translation o Happening in the same time RNA processing- eukaryotic nucleus  mRNA processing o 5’ cap = 7-methylguanosine added to the 5’ end o Poly (A) tail added at the 3’ end o Removal of introns and splicing together of exons Addition of 5’ cap  Very early in transcription process  Guanyltransferase enzyme adds guanosine to the 5’ end of mRNA  Must make a phosodiater bond o From three consecutive phosphates (triphosphate linkage) Practice problems: 2,3,4,8,9,11,12 Addition of a polymeric (poly) A tail  Endonuclease recognizes a sequence element o Polyadenylation signal “ AAUAAA” o Cuts the RNA transcript 3’ of this sequence  Anything further then the sequence element discarded as it is not needed  Once cleavage has occurred need enzyme that will add the ply a tail o New 3’ end when cut o Poly A polymerase will add the AAAAAAA..... Sequence Removing interrupting sequences fom mRNA  Unique to eukaryotic genes encoding proteins  Intervening seuqneces o Does not code for the functional protein  Introns- do not code o Removed after transcription  Exons= coding proteins o These sequences will be present in the final mature mRNA Splicing  Introns are transcribed along with exons in the primary transcript o RNA faithfully transcribes everything  Introns are removed and exons are spliced together. o Splicing is process of removing introns o Stitching the exons together  Splicing o Disrupting phospodiester bonds in between introns and exons o Then making new phosphodiester bonds with exons adding to exons  Must be extremely accurate or no functional protein can be made How are correct sires for splicing chosen?  Compared many genes- looking for trends in many different mRNA  Conserved sequences specify intr
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