b207p03.pdf

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Department
Biology (Biological Sciences)
Course
BIOL201
Professor
Michael Harrington
Semester
Fall

Description
Biology 207 - Practice Questions 3 Biochemical pathways & complementation tests Question 1 What is the purpose of: a) genes b) cell division c) mitosis d) meiosis e) eukaryotes alternating between haploid and diploid f) recombination during meiosis g) meiotic crossovers Question 2 Consider the experiment done by Srb and Horowitz (Lecture 5 section 3). a) The symbol '+' is used to represent three different things. What are they? b) Positive and negative controls are done so that we know that an experiment has worked the way it should. Which control(s) in the table allow them to know that they didn't make the following mistakes? Use a WT strain of Neurospora instead of the arg3 strain. - Use argH cells that were dead. Use MM+citrulline media that was actually toxic. Question 3 Students often confuse biochemical pathway analysis with complementation tests. To illustrate the differences consider this synthesis pathway from S. cerevisiae. You have isolated three mutant strains unable to synthesize compound “B”. The blocked step in each mutant strain is shown. Mutations 1 and 3 are in the same gene. Complete both tables with the expected results where + indicates growth and - indicates no growth. Also identify positive controls and negative controls. a) Biochemical pathway analysis results b) Complementation test results Question 4 (This is an old midterm question.) You have isolated five Neurospora mutant strains that are unable to synthesize an essential compound, C.
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