MICRB265 Lecture Notes - Penicillin Binding Proteins, Autolysin, Exponential Growth
17 Oct 2013
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Cell division: mine gets concentrated in the middle of the cell and causes the ftsz ring to form. Ring gets pulled through zipa molecule to start cell division. Peptidoglycan precursors brought past the cytoplasmic membrane by. Glycolytic bonds are broken to allow for the insertion of new monomers by autolysin. Transpeptidation occurs and penicillin binding proteins are involved. Age of culture: the older the culture, the longer the lag phase. Growth substrates: the transfer from complex medium to defined media extends lag phase because the bacteria now have to synthesize new enzymes. Since bacteria grows exponentially, some of the earliest doubling events may not be detectable and may just look like an extended lag phase. It"s hard to tell where exponential growth occurs. N = cell density at time t". G = generation time (or doubling time) N = # of generation in time t". When determining doubling time from a semi-log plot, remember to exclude the lag phase!
