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Class Notes for Microbiology (Biological Sciences) at University of Alberta

MICRB265 Lecture Notes - Lecture 2: Aureus, Carl Linnaeus, Archaea
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Lecture 2 In 1900, most fatal diseases were infectious diseases (influenza, pneumonia, tuberculosis). In 2008, mostly lifestyle diseases were fatal (cancer, heart problems) Two main reason for such change: Public health c...

Microbiology (Biological Sciences)
MICRB265
Brian Lanoil
MICRB265 Lecture Notes - Lecture 1: Edward Jenner, Smallpox, Microbiological Culture
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Lecture 1 Robert Koch Discovered the method that isolated a pure bacterial culture. Proved that diseases are caused by specific microorganisms Kochs Postulates the cornerstone of bacteriology 1. The suspected pathogen mu...

Microbiology (Biological Sciences)
MICRB265
Brian Lanoil
MICRB265 Lecture Notes - Lecture 4: Cell Membrane, Myxococcus Xanthus, Phototaxis

2.2 Gliding Motility Movement along a solid surface o Occurs without ____________________ o Slower and smoother movement o Requires contact with solid surface o Produce characteristic colony morphology: Figure 2.40: More t...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 4: Protein Filament, Bacterial Adhesin, Biofilm

Adhesion and motility structures are o Anchored in cell envolope o Located outside cell wall 1. Attachment to Surfaces Adhesion = attachment to a surface Adhesin: o Cell surface component that assists in attachment to a su...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 4: Antimicrobial Resistance, Biofilm, Phagocytosis

Biofilms: Mixed community of bacteria growing on a surface Adhere using a ________________________ Have a complex structure Why form biofilms? 1. Protection o Resist physical forces o Resist phagocytosis by immune cells o ...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 4: Flagellation, Motor Protein, Rings Of Saturn

Motility The ability of an organism to move under its own power o Requires energy o Only used by some bacteria o Advantange allows cells to reach new enviorments and resources Two main types: o Swimming with flagella o Gli...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 4: Negative Stain, Polysaccharide

1.2 Capsules and Slime Layers Found outside of cell wall Composed of polysaccharides Capsule: o Tightly organized matrix o Excludes particles o Can be seen by negative staining o Tightly attached to cell wall Slime Layer: ...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 4: Biofilm

Topic 4: Adhesion and Motility Structures Readings: Readings from Brock Biology of Microorganisms, Fifteenth Edition: Chapter 2, pages 4850, 5663 Chapter 20, pages 620622 Chapter 25, pages 758759 Corresponding readings fro...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 3: Active Transport

2.4 Transport Systems Role of transporter proteins: o Increase ________________________ of nutrients o Move solutes across membrane against concentration gradient o Accumulate high concentrations of nutrients that are ____...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 3: Phospholipid, Glycerol, Sterol

1. Cell Membrane _____________________ that separates the cytoplasm from the cells environment 2.1 Structure What are the components of the cell membrane? A. Phospholipid bilayer o Made of _______________________________ p...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 3: Gram Staining, Tetrapeptide, Electron Microscope

3.1 Bacterial Cell Walls Bacteria can be grouped based on differences in cell wall structure o Gram stain o Electron microscopy Figure 2.9: All bacterial cell walls contain _____________________________ o Polymer responsib...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 3: Lipopolysaccharide, Glucosamine, Lipid A

3.1B GramNegative Cell Wall Thin layer of PG Outer membrane containing lipopolysaccharide and proteins Periplasm in between membranes Figure 2.14: Periplasm: o Gellike fluid High _____________ content High ______________ c...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 3: Ethylenediaminetetraacetic Acid, Lipoprotein, Hydrophile

Functions of outer membrane: o Permeability barrier Prevents movement of both hydrophilic and hydrophobic compounds Proteins allow selective movement across OM Maintains periplasmic composition Protection from antibiotics ...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 3: Mycoplasma, Mycobacterium, Gram Staining

3.1C Acid-Fast Cell Wall Variation on Gram-positive cell wall Contains: o Inner layer of ___________ o Middle _____________________ layer o Outer layer of mycolic acid (________...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein
MICRB265 Lecture Notes - Lecture 3: Facilitated Diffusion, Mycoplasma, Antiporter

Biology 211 Topic Outline Topic 3: Bacterial Cell Envelopes Readings: Readings from Brock Biology of Microorganisms, Fifteenth Edition: Chapter 2, pages 39-48 Chapter 3, pages 76-77 ...

Microbiology (Biological Sciences)
MICRB265
Lisa Stein

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