MICRB265 Lecture Notes - Lecture 6: Aerotolerant Anaerobe, Superoxide Dismutase, Azide
25 Apr 2018
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Nutritional diversity: c sources: organic, inorganic (co2, energy sources: light, chemicals (organic, inorganic) Extremophiles: tolerance of environmental conditions: extreme habitats (e. g. salt, temperature, industrial applications, o2 requirement and tolerance, review of electron transport and chemiosmosis, you are expected to know this material. Motive force (pmf) by setting up ph (h+ ion concentration) and ionic charge differentials across the cell membrane. For example, e. coli lacks cytochrome c, and therefore is oxidase negative (lab ex. Under anaerobic conditions in the presence of nitrate, it produces a different terminal cytochrome than under aerobic conditions. Under anaerobic conditions in the absence of nitrate (e. g. , in the gut) it ferments: fermentation reactions occur in the cytoplasm, and do not involve cytochromes, membranes, or creation of pmf. Therefore fermentation yields much less energy than either anaerobic or aerobic respiration: toxic oxygen species: biochemical reactions produce a number of reactive oxygen species, such as: 2 o2- (superoxide anion) + 2h+ (proton) h2o2 (peroxide) + o2.
