MICRB265 Lecture Notes - Lecture 16: Tetrapeptide, Cell Membrane, Glycan

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Tryptophan and glucose are difficult to transport active transport may be required. Chloride ions, potassium ion, sodium ion are very difficult to transport active transport required. Gram positive; monoderm phyla"s firmicutes and actinobacteria. have a thick layer of. Gram negative diderm, have an outer layer all other known phyla. have a thin layer of peptidoglycan. one membrane around cytoplasmic membrane. peptidoglycan. two membranes (cytoplasmic membrane and outer membrane). Cells stained uniformly with insoluble crystal violet-iodine complex. Cells are decolorized with alcohol gram +ve peptidoglycan is dehydrated. Gram -ve bacteria are counter stained with safranin and become pink. Examples: gram +ve bacillus cereus (note this species is pleomorphic) . gram -ve . Gram +ve and -ve have pg (peptidoglycan) linkages. Rigid layer that provides strength (more strength than outer layer) composed of; Alternating modified glucose (n-acetylglucosamine and n-acetylmuramic acid) in b-1,4. Amino acids l-alanine, d-alanine, d-glutamic acid, and either l-lysine or diaminopimelic.

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