MICRB265 Lecture Notes - Lecture 4: Agarose Gel Electrophoresis, Messenger Rna, 5.8S Ribosomal Rna
1 May 2019
School
Department
Course
Professor
Document Summary
60+ genes including rrna shared by all cells present in common ancestor. Eukaryotic and archaeal genes are more similar. Divergence of bacteria and archaea before the existence of eukaryotes. Luca: dna genome, could transcribe and translate, prokaryotic. Many genes shared by organisms belonging to two of the three domains because of horizontal gene transfer: rnas trna: transfer rna translation attaches to amino acids and brings them to ribosomes. Mrna: messenger rna encodes amino acid sequence of a protein rrna: ribosomal rna forms complex with ribosomal protein the part that catalyses translation. Small sub unit rrna: ssy rrna: 16s rrna (30s protein): bacteria, archaea, mitochondria and chloroplasts, 18s rrna (40s protein): eukaryotes. Large sub unit ribosomal rna : lsu rrna: 5s +23s rrna (50s protein): bacteria, mitochondria, chloroplast, archaea, 5s + 5. 8s + 28s rrna (60s protein): eukaryotes, detect and identify 16s rrna from an environmental sample. Run on agarose gel check for correct size.
