MICRB315 Lecture Notes - Lecture 2: Bioprospecting, Microbiological Culture, Recombinant Dna
18 Oct 2015
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Document Summary
Refers to improving the producing strain and/or host for recombinant protein production and/or cloned genes encoding product or pathway for producing product. Some of the desirable traits can be improved by genetic manipulation; others can be controlled by improved growth conditions rather than strain improvement: Some specific desirable traits: high yield of desired product (biomass or metabolites, safe to grow and handle (non-pathogenic, grows in liquid culture in desired morphology. Shape of pellet ( a vs b vs c) - changes expression of genes and pathways. Contamination potential m o c s a l i t n e s s e o c y m. Expense of aeration/agitation, possibly filtering air input: low culture viscosity. Expense of aeration/agitation, possibly ltering air input low viscosity: In uences energy requirements for mixing, o2 deliver, foaming. Easier to harvest later cell lyse easily. For ease of strain improvement, easier downstream processing (product recovery and puri cation)
