MICRB315 Lecture Notes - Lecture 6: Novozymes, Immobilized Enzyme, Biocatalysis
18 Oct 2015
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High fructose syrup (hfs) production two enzymatic routes invertase a. b. c. amylases (again) d. Glucose isomerase" huge business b/c of soda pop. 1. catalytic proteins; may require metals and/or organic co-factors to function or inorganic. 2. accelerate spontaneous reactions but cannot contravene thermodynamics cant make a reaction occur if it doesnt occur. 3. are not consumed by the reaction but they do. Wear out" (i. e. , proteins degrade with time) enzymes are proteins -> they do break down, have half life. 4. can be extremely specific regarding reactants and products, or can have relaxed specificity. 5. have optimum conditions for reactions: e. g. , temperature, ph, [substrate], metals and co- factors, sensitivity to poisons" and inhibitors (these are approximately the same as the tolerances of the organisms that make the enzymes) typically require an aqueous phase. To maintain their 3d structure (there are other ways to make it use less water) There are products that diffuse from active site.
