CMMB 421 Lecture Notes - Lecture 10: T7 Rna Polymerase, T7 Phage, P1 Phage

• School: University of Calgary
• Department: Cellular, Molecular and Microbial Biology
• Course: CMMB 421
• Professor: Michael Hynes

Document Summary

The family podoviridae consists of phages with short stubby non-contractile tails. Their genomes are made of dsdna and are 20 100 kb. Dna is pulled into cell by host rna polymerase, then highly specific phage encoded rna polymerase. Terminal direct repeats at end of phage genome allow concatemer formation. Processing of concatemers during packaging regenerates intact repeats at end of genome. Stage i genes are transcribed by e. coli rna polymerase, stages ii and iii by phage encoded rna polymerase. T7 phage genome encodes a number of genes involved in subverting host defenses and. Interfering with host metabolism (antirestriction factors, endonucleases, exonuclease. Phage lands on lps, ejects stage 1 genes that go through periplasmic space and im. Dna is grabbed by host rna pol and stage 1 genes are pulled through which are transcribed. Genome is replicated from origin of replication that is close to left side of dna.