CMMB 421 Lecture Notes - Lecture 11: Shiga Toxin, Rolling Circle Replication, Caudovirales
28 Dec 2019
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More different subfamilies than the other families in the caudovirales. E. coli temperate phage lambda ( ) is the best studied example, and one of the best known and characterized systems in all of biology. We owe discovery of restriction enzymes, operons, repressors, lysogeny, termination and many other things to the study of , and have also developed tools like phage cloning vectors, packaging mixes, cosmids, recombinases (red, int) etc. Majority of phages are temperate, we just don"t see them because their activity isn"t as noticeable as that of lytic phages. Long flexible tail with fibres at the end involved in injection. Lambdoid phages = close relatives, include shiga toxin encoding phage of e. coli, p22, Head is a naked icosahedral capsid (t=7), 63 nm diameter. Flexible, tube shaped tail, 135 nm long. Lambda infects, penetrating through om and im to inject dna into cytoplasm of e. coli. Replication of phage genome, then lyse cells.
