CMMB 511 Lecture Notes - Lecture 1: Reverse Transcriptase, Gene Knockout, Crispr
28 Dec 2019
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Historical development of the field of molecular biology. Reverse transcribed into cdna using reverse transcriptase. Dna, over 50 years used the methods below: Rnai: hybridize rna with cdna to down regulate because can"t make proteins since. Idea is to link all -omics, none of the -omics are enough by themselves, so need to find a way to link all of them. Marker development can be done when proteomics and metabolomics are linked. Nucleic acids in linear structure don"t reveal function, so need to look at secondary structures as well. To determine which genes are active for which function. Use various methods and approaches to reach results. These are important questions in the understanding of cellular processes including: Changes and gene expression are very important in this stage. Discovery of recombinant dna technology and others. Three questions in the studying gene control: Once signal is there, there are changes in expression levels.
