ANSC 4050 Lecture Notes - Lecture 4: Protein Folding, Transgenesis, Agarose Gel Electrophoresis
18 Sep 2017
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Document Summary
Vectors: able to accept foreign dna fragment, can be replicated inside the host, examples, plasmids, cosmids and bacteriophages. Islation and collection mrna and using recerse transcription to make dna. Insertion into bacterial plasmids: plasmid insertion in to bacteria and screening. Gene therapy: goal to change the genetic material of a patient"s cells for therapeutic purposes (treating a genetic disease, the genetic material introduced either, replaces a defective protein, gene editing for correction of mutations. Integrase: the product of the vpr gene, matrix (product of the gag gene, the complex enters the nucleus to influence gene expression. Transient transfection: expression construct is circular (higher transfection efficiency, no need for selection, plasmids not integraed into genome but is staying in the genome and the cell gets rid of it, is there enough to do its job. Stable transfection: expression constructs are linearlized, selection is needed to get stable transformants, plasmids can integrate into genome. Neomycin, resistant gene, reporter gene: protein purification component.
