MICR 2420 Lecture Notes - Lecture 5: Shower, Winogradsky Column, Metagenomics
28 Mar 2016
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Document Summary
A plate count is what you spread in a plate, it"s a technique for getting an idea how many organisms are in the sample. Wherever find fermentative organisms you will find acid. Community < guild: guild = group of different kinds of organisms that do the same thing, have the same ecological niche, communities are a group of organisms ex. Aerobic organisms are the top of the winogradsky column. Identifying the uncultivated: norman pace, father of metagenomics and his graduate students, studied the phylogeny of a shower curtain biofilm. Came up with a way to purify dna straight from the environment, bypassing the culturing process which is sometimes a problem. Using fluorescence microscopy, enables them to see how many organisms are in the sample because the dye targets anything with. Metagenomics of a shower curtain (done by students) identified 117 unique ssu rrna (small subunits) sequences, many of which had never been identified.
