MBIO 1010 Lecture Notes - Lecture 13: Flow Cytometry, Microbiological Culture, Chemostat
8 May 2017
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Interval between inoculation of a culture and beginning of growth (i. e. increase in cell numbers) Cells in this phase are typically in the healthiest state. Cells metabolically active, but growth rate of population is zero. Either an essential nutrient is used up, or waste product of the organism accumulates in the medium. If incubation continues after cells reach stationary phase, the cells will eventually die. Not all bacteria die, some bacteria form spores/cysts or dormant stages that allow a significant proportion of cells to survive for a long time. Continuous culture an open-system microbial culture of fixed volume. Both growth rate and population density of culture can be controlled independently and simultaneously. Dilution rate: rate at which fresh medium pumped in and spent medium is pumped. Concentration of a limiting nutrient controls the population size and the growth rate. is. Microbial cells can be enumerated by direct microscopic observations using a petroff-
