MBIO 1010 Lecture Notes - Lecture 4: Dark Field Microscopy, Bright-Field Microscopy, Fluorescence Microscope

• School: University of Manitoba
• Department: Microbiology
• Course: MBIO 1010
• Professor: Christopher Rathgeber

Document Summary

The refractive index (cid:271)e(cid:374)ds light to a (cid:272)ertai(cid:374) degree allows you to generate a live sample with a large contrast between internal structures. Dark field microscopy : specimen is illuminated with a hollow cone of light (fig. 2. 5c) o only refracted light enters the objective: specimen appears as a bright object on a dark background, used to observe bacteria that don"t stai(cid:374) well, ex. Treponema pallidum the causative agent of syphilis. The background appears (inverse of bright field microscopy) Understand between the different microscopy the utility of them and how they look (dark background) not how the microscope is doing it. Fluorescence microscopy: used to visualize specimens that fluoresce, emit light of one color when illuminated with another color of light, cells may fluoresce naturally, ex. Emits at 670 nm (red: or after staining with fluorescent dye ex. The portion of the antibody is specific to molecule of interest.