MBIO 2020 Lecture Notes - Lecture 6: Uracil, Pribnow Box, Escherichia Coli

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grayrabbit360

10 Nov 2016

School

University of Manitoba

Department

Microbiology

Course

MBIO 2020

Professor

Dibrov Pavel

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Document Summary

Gene - a dna segment that codes for a single polypeptide, rrna, or trna. Rna pol binds to the specific region in dsdna called promoter about 29-200 nucleosides. Promoter sequence lies upstream/ahead of actual transcription starting bp - +1bp - of any gene. The promoter itself is not transcribed - only function is to bind the transcriptase. Rna pol recognizes a certain sequence located 35 bp upstream of +1 - rna recognition site, It binds to another specific sequence rna binding site located at the -10 element position 5"- Tataat-3" also called pribnow box or -10 element. Both sequences are on coding strand - complementary to template strand. They are consensus sequences - most common promoter sequence. Even if -10 element is intact - if -35 element isn"t - transcription wont happen. The sigma factor positions the holoenzyme at the -10 element and core enzyme starts to unwind.

Related Questions

Please help on these. Thank you!

Question 1

Which of the following is NOT a channel in the RNA polymeraseenzyme?

		RNA exit channel
		DNA entry channel
		DNA exit channel
		Nucleotide exit channel

Question 2

A promoter region upstream of a bacterial gene typically has thesequence TTGACA in the -35 position and TATAAT in the -10 position.If there was a 5 bp insertion at the -40 position, such thateverything downstream was shifted (ie: the "-35 element" is now atthe -30 from the transcription start site), which of the followingwill occur?

		Sigma protein will not bind and so no transcription willoccur.
		RNA polymerase will not bind and so no transcription willoccur.
		Sigma protein will bind to its target sequences and sotranscription will start 5bp downstream from the originaltranscription start site.
		Sigma protein will bind as usual and transcription will startappropriately at the transcription start site.

Question 3

A person has a mutation that prevents TFIIH from being produced.Which of the following outcomes will occur?

		No transcription because TFIID will not be able to bind.
		No transcription because the DNA strand will not be opened.
		Transcription will occur, but it will not be in the correctposition.
		Transcription will occur as usual.

Question 4

There are 4 rRNAs produced to make both subunits of theribosome. How many transcripts are required to produce these 4rRNAs?

		1
		2
		3
		4

Question 5

True or False: the 5' 7m-Gppp cap is attached to the start codonof the mRNA.

		True.
		False, the cap is attached to the 5'-untranslated region.
		False - the cap is attached to the 3'-untranslated region.
		False, the cap is attached to the polyA tail.

Question 6

During the splicing mechanism, which of the following joins twoexons together?

		A 2'OH attacks a 5' phosphate
		A 5' phosphate attacks a 2'OH
		A 3'OH attacks a 5' phosphate
		A 5' phosphate attacks a 3'OH Question 7 After their experiment in Neurospora, Beadle and Tatum concludedthat each single genes code for single enzymes. Do you agree withtheir statement, why or why not?

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MBIO 2020 Lecture Notes - Lecture 6: Uracil, Pribnow Box, Escherichia Coli

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