MBIO 2420 Lecture Notes - Lecture 12: Tobacco Mosaic Virus, Sybr Green I, Exponential Growth
7 Dec 2016
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Used for real time for sybr green. Goes up from 55 - 95 degrees. Measures the florescent - once it unbinds from dna the florescence stops: on graph - when its going down - dna is denaturation. The derivative of the graph gives a rate of change: the point of the peak is the melting point. If there are two peaks - two products - bad sample. Tobacco mosaic virus: late 1800s - ivanovsky and beijernick. First viruses described: crush tmv infected leaves (tobacco plant) and filter them, an agent in the filtrate could be rubbed on a new leaf and infect once again. Filter blocks bacteria from going through - viruses can though. Tb bacteria is like a virus that it lives inside a cell. Viruses require a cell to replicate - they are not free living - will not grow on media. Virus is added - adsorbed: latent period, exponential growth, then release.
