MBIO 3410 Lecture Notes - Lecture 5: Rolling Circle Replication, Non-Coding Rna, Viroid
24 Dec 2015
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Viroids & virusoids: naked ssrna, doesn"t code for anything, uses host rna polymerase and replicates by rolling circle replication, parasitic and infectious, viroids spread by mechanical means. E. g. insects biting plants: virusoids get carried around with other viruses. Usually found in plants ssrna molecules (tandem repeats) E. g. pear blister canker viroid, potato spindle tumor: can also cause human infections viroid. Hepatitis b particle with the satellite rna. Need host rna polymerase ii for replication: rolling circle replication. Some are ribozymes (hammerheads: can self-splice into monomers, rna with catalytic properties, rna forms to fold hammerheads, cuts and ligates itself to recircularize (cid:1) Molecules of a normal body protein that have changed their 3d. Prions are resistant to proteases and high temperatures: can survive autoclave conditions, harsh solvents/denaturants configuration (high ph) Transmitted by prion-contaminated tissue from another animal. N-terminal regions important: if n-terminal regions are similar, human proteins can be corrupted.
