MBIO 4600 Lecture Notes - Lecture 10: Wild Type, Arginine, Chromosome
25 Dec 2015
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Strategy of mapping is similar for both transformation and transduction. Conjugation relative order and distance of genes. Transformation/transduction determine distance between genes located close together. Based on genetic markers being carried on the same piece of dna: selected marker and unselected marker, co-transformed or co-transduced . Percentage of the total transformants or transductants selected for one marker that are also recombinant for the other marker: co-transformation or co-transduction frequency, the higher the frequency, the closer together the two markers. Can estimate distance between markers on e. coli dna: recall that chromosome is 100 min long (4500kb, p1 phage head can only hold 2% of that length, so markers are less than 2 min (90kb) apart. If you want to know the order of argh5 and rif-8 markers within. Only certain genes close to the attachment site of a lysogenic phage in the chromosome can be transferred: transducing phage will carry both bacterial genes and phage genes.
