BIOL303 Lecture Notes - Lecture 10: Blastoderm, Syncytium, Zebrafish
3 Oct 2017
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Early zebrafish development: ~trivia~ zebrafish sperm lack an acrosome, )e(cid:271)(cid:396)afish is a good (cid:862)ge(cid:374)eti(cid:272)(cid:863) o(cid:396)ga(cid:374)is(cid:373) Microinjection of blastomeres permits cell labeling experiments or transgene expression: this was the first vertebrate system extensively studied by mutagenesis screens, mutations have been isolated in different genes, undergoes meroblastic cleavage. Large scale genetic screens have been carried out. Random mutagenesis where a mutagenized individual is crossed with a wild-type. Recovery of a single male carrying a chromosome that was exposed to the mutagen. In f2 the mutated chromosome is carried by 50% of all progeny. 1 in 4 random matings will potentially uncover any recessive lethal mutation in generation. Like humans, the oocyte completes meiosis upon fertilization. Undergoes prophase i arrest to metaphase ii arrest: zebrafish primary oocytes are highly transcriptionally active during the growth phase. Transcriptional activity during the diplotene stage of meiosis results in cytologically distinctive lampbrush chromosomes.
