BIOL308 Lecture Notes - Lecture 14: Aspergillus Nuclease S1, Repressor, Rifampicin
20 Jun 2017
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Document Summary
*slides 1 to 12 is covered in the previous lecture notes document. One strand of dna is used as a template for complimentary mrna (non-template = coding = sense = rna like) Multi-subunit enzyme makes a strand in 5" to 3" with mg2+ as a cofactor and both dna strands to be a template. Rnap: doesn"t need a primer (rntp precursor), has asymmetrical binding sites in the promoter so it only transcribes one strand (+ or -) Atp not required since dna unwinds locally and can undergo energetically favourable structures. Promoter and rnap undergo a confirmation change, the open complex/bubble opens and rnap makes about 10 bases. Dna is unwound in front of rnap and annealed behind it to continue polymerization and proofreading and dissociating the rna chain. Promoters are located near the gene"s transcription start site (which is the 3"oh of the promoter) Cis regulatory elements are up (in 5"utr) and downstream (in 3"utr)
