BIOL308 Lecture Notes - Lecture 8: Crosslinking Of Dna, Cdc6, Proliferating Cell Nuclear Antigen
4 Feb 2015
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Fyi: laptop multitasking hinders classroom learning for both users and nearby peers. Also removes rntps (including those directly linked to the dna) and replaces them with dntps as it has available 3"oh group and template!! 5"-3" exonuclease removes ribonucleotides in front of it. 5"-3" polymerase replaces removed rntps and fills the gap with dntp. 3"-5" exonuclease (proofreading not on this fig. ) Free 3 oh necessary for polymerization removes faulty nucleotides and fills the gap (as it has a template) removes faulty nucleotide in 3"-5". Enzyme that links fragments on the lagging strand. 3"-oh terminus and the 5"-po4 terminus of adjacent nucleotides. Topoisomerase ii (gyrase) requires atp introduces negative supercoils around oric-necessary for initiation of replication by dnaa. After helicase"s action positive supercoils are formed ahead of a growing fork; topoisomerase. Two dna pol iii cores are connected by dimers. Leading strand core elongates leading strand from rna primer in direction of growing fork.
