BIOL308 Lecture 2: Lecture 2

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Biochemical approach start from the protein (which is isolated by/through its function or activity) go towards dna. Genetic approach start from the gene - dna (normal vs. mutated) go towards production of protein (normal vs. mutated) Links between (or tools for both) genetic and biochemical approaches: recombinant dna technology bioinformatics. Genetic approaches: protein to gene gene to protein. Isolate and purify protein of interest based on its function or activity (example: rna polymerases) The protein activity could be assayed under different conditions (confirmation of identity, purity, activity) The ultimate goal: amino acid sequence is used to deduce the nucleotide sequence of the gene which codes for extracted protein. Genetic approach enables us to get the answers to following questions: what is a potential function and importance of the protein? through identification / isolation / survival of. What is the order of these genes (proteins) in the process? by applying genetic approach called.

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