BCH 3120 Lecture Notes - Lecture 5: Farnesyl Pyrophosphate, Farnesyl-Diphosphate Farnesyltransferase, Squalene

• School: University of Ottawa
• Department: Biochemistry
• Course: BCH 3120
• Professor: Elaine Beaulieu

Document Summary

Also produced in intestines, adrenal glands and reproductive organs: all cholesterol"s carbon atoms derived from acetate which we get from acetyl-coa (tricarboxylate transport system) Condensation of acetate to form mevalonate intermediates (c5 unit: i) from acetyl-coa (c2) to mevalonate (c6) Enzymes involved in forming hmg-coa that"s needed for ketone bodies are in mitochondria. Enzymes needed for forming hmg-coa for cholesterol are in cytosol and peroxisomes. Hmg-coa reducatase catalyzes rate limiting step of cholesterol biosynthesis and is an integral membrane protein. Can also be found in er and peroxisome: ii) from mevalonate (c6) to dmapp (c5) Then the reaction is spontaneous to from activated ipp then catalyzed by ipp isomerase to form activated. Isoprenoids: any compound biosynthesized from or containing isoprene units: need 3 acetyl-coa to produce 1 mevalonate and also need 3 to produce ipp. Polymerization of isoprenoids (c5) to form squalene (c30 unit: reactions 1 and 2 are catalyzed by prenultransferase.