BIO 1140 Lecture Notes - Lecture 1: Okazaki Fragments, Single-Strand Dna-Binding Protein, Primase
8 Nov 2016
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#1 the following dna molecule needs to be replicated. Which enzyme would replicate the first few nucleotides and what would be the obtained sequence: dna polymerase; taat, dna polymerase; gggg, primase; taat, primase; gggg, none of the above. #2 list and explain the steps, supporting molecules and enzymes involved in replicating linear eukaryotic dna. (7 marks) Ligase: closes the gap between the fragments. Ssbp: attach to nitrogenous base in order to stabilize, prevents recoiling. Topoisomerase (syrase): prevents supercoil: rna primase. Starts: dna polymerase iii + sliding clamp. Elongates leading strand: dna polymerase i. #3 replicating ends of a linear chromosome creates a problem at each replication cycle. Explain what the problem is and provide details of the mechanism that organisms use to resolve it. Problem: structure: rna primer (at telomeric region of the template strand) is removed, function: single stranded region is quick to degrade and shortens at the end of every replication cycle= loss of genetic material and genes.
