BIO 1140 Lecture Notes - Lecture 15: Prokaryote, Polysome, Signal Recognition Particle

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champagnetiger480

24 Mar 2017

School

University of Ottawa

Department

Biology

Course

BIO 1140

Professor

Caroline Petit- Turcotte

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Document Summary

Assembled in cytosol when binding to mrna (small to mrna then larger completes ribosome) A site: aminoacyl-trna arrives with proper amino acids. Met is the first aa and is the only one that goes straight to the p site. Mrna is recruited and large subunit then completes the ribosome. On the most recent trna in the p site. An aminoacyl trna binds to the a site, escorts by ef-tu bound to gtp. A peptide bond is formed between the carboxyl group of met at the p site and the amino group of the newly arrived amino acid at the a site. A site empty, and elongation factors with a trna goes to the a site and releases, peptidyl transferase binds carboxyl group of aa 1 with amino acid aa 2. trna discharges and its empty. Translation is terminated when stop codon arrives at the ______ site. Stop codon reached; not trna with that anticodon.

Related Questions

Facts about ribosomes

Indicate whether each of the following statements aboutribosomes is true or false.

1. Ribosomes are composed of tRNA and protein. TrueFalse
2. During translation in eukaryotes, some ribosomes remain free inthe cytoplasm while others attach to protein channels embedded inthe RER. TrueFalse
3. During initiation of translation, the small ribosomal subunitjoins to the mRNA and initiator rRNA, followed by the largeribosomal subunit. TrueFalse
4. When a ribosome first assembles on the mRNA, the initiator tRNAoccupies the A site while the P and E sites are empty.TrueFalse
5. During translation, a charged tRNA first binds to the ribosomeif its anticodon matches the codon in the E site. TrueFalse
6. A peptide bond forms between the amino acid attached to a tRNAin the A site and the growing polypeptide attached to a tRNA in theP site. TrueFalse
7. Uncharged tRNA molecules in the E site are ejected from theribosome. TrueFalse
8. No more than 2 tRNA molecules are bound to a ribosome at anygiven time. TrueFalse
9. During translocation of the ribosome, tRNA molecules move fromthe P site, to the A site, to the E site, and are then ejected fromthe ribosome. TrueFalse
10. During translation, an amino acid attached to a tRNA in the Asite is transferred to a growing polypeptide attached to a tRNA inthe P site. TrueFalse

periwinklemuskrat632

Facts about ribosomes

carmineraccoon103

Gene expression is a multi-step process. Place these events in the correct order.

Question 13 options:

charged tRNA molecules read the next codon, entering the A site of the ribosome.

Translocation of the mRNA to the cytoplasm.

Recruitment of RNA polymerase.

tRNA(Met) binds to the start codon AUG in the P site of the ribosome.

Assembly of small and large ribosome subunits on the mRNA.

Processing of the primary RNA molecule.

Release factors recognize the stop codon, signaling the release of the protein, mRNA and ribosome subunits.

Synthesis of primary RNA molecule, a direct copy of the DNA template.

Recognition of the mRNA by the small ribosome subunit .

Peptidyl transferase, transfers the amino acid of the incoming tRNA to the growing peptide chain.

Binding of transcription factors to the promoter.

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BIO 1140 Lecture Notes - Lecture 15: Prokaryote, Polysome, Signal Recognition Particle

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