BCH 3170 Lecture Notes - Lecture 19: Rna Splicing, Phosphodiester Bond, Exon Skipping
2 Mar 2016
School
Department
Course
Professor
Document Summary
Control of gene expression in eukaryotes: maturation of mrna. Occur when about 20-30nts have been synthesized. Fairly simply, involves three enzymes and three steps. Alpha beta and gamma phosphates on gtp (closest to furthest) Phosphatase comes and removes the gamma phosphate, so the terminal will be a ribonucleotide diphosphate. Next a guanyl transferase comes and removes beta and gamma to make a. This gmp is then transferred to the rndp through an inverse phosphodiester bond, 5" to 5". A ribonucleotide methyl transferase will add a methyl group at position 7 of guanosine to protect it. Distinguish transcripts produced by the rnap ii from those produced by the other two rnaps. Facilitate interaction with the cap binding complex, essential for processing transport and translation. 5" splice site or donor site" is the key sequence you need. There"s also a branch site and a 3" splice site or acceptor" site.
