BPS 3101 Lecture Notes - Lecture 7: Fluorescent Tag, Hybridization Probe, Oligomer

• School: University of Ottawa
• Department: Biopharmaceutical sciences
• Course: BPS 3101
• Professor: Linda Bonen

Document Summary

Assigning gene function by experimental analysis: 1. Gene inactivation (loss-of-function: mutate gene (knock out) and observe change in phenotype. 2. (i) deletion mutagenesis, by homologous recombination: deletion cassette vector . Substituted dna can have selectable marker, restriction sties, 6. (iii) rna interference: short, antisense rnas (21-25 nt length) in hybrid with specific mrna triggers degradation, knock-down of gene expression, gene is still intact; mrna is prevented from making the protein. Gene of interest between promoters for t7 phage rna polymerase. To generate ds rna in vitro and then introduce into organisms of interest. Dicer nuclease cleaves the introduces ds rna into short sirnas, sirnas then attach to target mrna degradation by rde-1. Study of 2769 c. elegans genes on chromosome 1. Pcr product is desired we can use the green and blue oligomers. How to determine where and when a gene is expressed: 1.