HSS 1100 Lecture 3: 3HSS1100
25 Feb 2017
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Pick which one is true or false, there are usually two choices that are close. Short term - store in fridge 4 degrees, 2-3 months. Want to keep bacteria for research purposes. Colony morphology: look at colony of bacteria. Inoculated plate, stored it and see a colony growing: look at form, elevation and margin. Cellular morphology: use a microscope to view an individual bacterial cell. Run it thru bunsen burner multiple times without burning it. Can"t buy a bottle of gram stain. Bacteria in a sample, prepare a smear. Flood stain with crystal violet - dark purple, let it sit a bit : stains peptidoglycan, will bind more in a gram positive. At the end of time wash it with water and drain it. Now flood the stain with iodine solution - does(cid:374)"t (cid:272)ha(cid:374)ge (cid:272)olou(cid:396) just helps the (cid:271)i(cid:374)di(cid:374)g (cid:271)e (cid:373)o(cid:396)e tight.
